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Biosphere 2 Project founder John Allen with map of the planet Mars. Allen is pointing to what he thinks is a probable landing/colony site on the Mars map. Biosphere 2 was a privately funded experiment, designed to investigate the way in which humans interact with a small self-sufficient ecological environment, and to look at possibilities for future planetary colonization. The $30 million Biosphere covers 2.5 acres near Tucson, Arizona, and was entirely self- contained. The eight ‘Biospherian’s’ shared their air- and water-tight world with 3,800 species of plant and animal life. The project had problems with oxygen levels and food supply, and has been criticized over its scientific validity. 1990